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hand2  (Santa Cruz Biotechnology)


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  • 93

    Structured Review

    Santa Cruz Biotechnology hand2
    Hand2, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hand2/dHAND+Antibody/pm41707997-181-63-64
    Average 93 stars, based on 18 article reviews
    hand2 - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Incubation:

    Article Title: Menin directs regionalized decidual transformation through epigenetically setting PTX3 as an obstacle balancing FGF and BMP signaling
    Article Snippet: .. Uterine slices were deparaffinized and incubated 573 in citrate buffer for antigen retrieval by hyperbaric heating and then incubated 574 overnight at 4°C with the primary antibodies including Menin (Bethyl, 1:1000), 575 COX2 (Santa Cruz, 1:200), Dtprp (homemade, 1:200), Ki-67 (Abcam,1:200), 576 PL1 (Santa Cruz, 1:200), 3β-HSD (Santa Cruz, 1:200), p450scc (Santa Cruz, 577 1:200), PR (CST, 1:200), HAND2 (Santa Cruz, 1:200), p27(abcam,1:100), pH3 578 (CST, 1:200), ERK1/2 (CST, 1:200) and p-ERK1/2 (CST, 1:200). .. A Histostain-579 SP Kit (Zhongshan Golden Bridge Biotechnology) was applied to visualize the 580 antigen.

    Article Title: The protein phosphatase 2A-B56α complex regulates N-Myc degradation in neuroblastoma.
    Article Snippet: PageRuler Prestained Protein Ladder (Fisher Scientific, PI26617) was used to confirm expected kDa of targets (Fisher Scientific, PI26617). .. Membranes were blocked in 5% non-fat milk/TBST for 1 h and incubated overnight at 4°C with primary antibodies: c‐Myc (ABclonal, A19032, 1:1000), phospho S62 c-Myc (Abcam, ab185656, 1:1000), N‐Myc (Abcam, ab16898, 1:1000), Vinculin (Santa Cruz Biotech, sc‐73614, 1:5000), GAPDH (Santa Cruz Biotech, sc‐32233, 1:2,000), V5-tag (Cell Signaling Technology, 13202, 1:1000), AP-2 beta (Cell Signaling Technology, 2509, 1:1000), GATA3 (Cell Signaling Technology, 5852, 1:1000), HAND2 (Santa Cruz Biotech, sc-398167, 1:1000), PHOX2B (Santa Cruz Biotech, sc-376997, 1:1000), PARP (Cell Signaling Technology, 9542L, 1:1000), Cleaved caspase‐3 (Cell Signaling Technology, 9664L, 1:1000), and PPP2R2A (Santa Cruz Biotech, sc-81606, 1:1000). ..

    Western Blot:

    Article Title: EP300 selectively controls the enhancer landscape of MYCN- amplified neuroblastoma
    Article Snippet: Chromatin lysates were prepared with the total histone extraction kit (Epigentek). .. Briefly, equivalent amounts of protein were resolved by western blotting using 4–12% Bis-Tris NuPAGE gels (Thermo-Fisher Scientific) prior to transfer and immunoblotting using primary antibodies to: MYCN, total H3, CBP, Cas9, cleaved-PARP1, cleaved Caspase-3, β-actin TFAP2β, CRBN, H3K27ac, EP300, GATA3, Vinculin and HAND2 (Santa Cruz Biotechnology) RRIDs: RRID:AB_2800038, RRID:AB_2756816, RRID:AB_2616020, RRID:AB_2750916, RRID:AB_2160739, RRID:AB_2341188, RRID:AB_330288, RRID:AB_2058198, RRID:AB_2799810, RRID:AB_2118291, RRID:AB_297224, RRID:AB_11212253, RRID:AB_309711. .. Secondary antibodies were HRP-conjugated anti-rabbit or anti-mouse (Santa Cruz Biotechnology), incubated prior to exposure to enhanced chemiluminescence reagents (GE Amersham).



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